quant it picogreen dsdna assay kit. Replicates of four were added to a 96-well plate using 2 μL volumes for rows A through D (beige) and 10 μL volumes for rows E through H (blue). quant it picogreen dsdna assay kit

 
 Replicates of four were added to a 96-well plate using 2 μL volumes for rows A through D (beige) and 10 μL volumes for rows E through H (blue)quant it picogreen dsdna assay kit  Thermo Fisher quant it picogreen dsdna assay kit

Figure 8. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. Libraries were quantified using the Quant-iT PicoGreen dsDNA Assay Kit (Life Technologies) and the Kapa Illumina GA with Revised Primers-SYBR Fast Universal kit (Kapa Biosystems). 50 pg to 2 µg. If your are using plastic cuvettes or assay plates, reduce this problem by including 0. Catalog Number. DNA samples with a concentration determined by 260 nm absorbance higher than 100 ng/mL were diluted. For duplicate-well droplet. According to the obtained results, the assay presents a linear correlation in a wide range of 0. クイックオーダー. Picogreenアッセイは、ほとんどのマイクロプレートリーダーおよび蛍光光度計に. Picogreenアッセイは、ほとんどのマイクロプレートリーダーおよび蛍光光度計に対応します。. 5), 200X PicoGreen. Picogreen 定量试剂盒兼容大多数微孔板读数仪和荧光计。. The AccuBlue® High Sensitivity assay is also available without any DNA standards ( 31008 ), for those that wish to use their own standard. (Center) The Quant-iT RiboGreen RNA Assay provides linear quantitation. Quant It Picogreen Dsdna Assay Kit, supplied by Thermo Fisher, used in various techniques. Materials: 1. The Quant-iT™ PicoGreen® DNA assay is the oldest assay and the most general-purpose. ” J Pharm Biomed Anal. Quant-iT™ PicoGreen ® dsDNA Assay Kit. The PicoGreen assay has high specificity for dsDNA versus RNA or ssDNA, and is. 0 fluorometer—provides state-of-the-art reagents for sensitive and selective quantitation of DNA, RNA or protein samples using a standard fluorescence microplate reader or the Qubit 2. The assay is a homogeneous, mix-and-read format where the fluorescence is proportional to analyte present. Selectively detect dsDNA from a wide variety of sources, and even in presence of. 2023-11. 1 ng/μL to 2000 ng/μL, with an extended upper range to 4000 ng/μL. All NGS libraries generated with both the Fragmentase and the FTP method contained similar amounts of ds DNA (800 ± 50 ng) and had similar mean insert sizes of the libraries in a range from 400 to 500 bp. Cloning, expression and proteins purification. 2. Library quality and average fragment size was assessed using a Bioanalyzer (Agilent, G2939A) High Sensitivity DNA chip (Agilent, 5067-4626). 2–100 ng: 2-1,000 ng: 0. 0). Pico488 allows to quantitate as little as low pg/mL of dsDNA. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. 3 Q33120 39. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. Double stranded DNA quantification performed in down to 2 µl using the LVis Plate. Before using PicoGreen dye to stain DNA, the concentrated Me 2 SO solution containing PicoGreen dye was diluted 200-fold using 1× TE buffer to prepare 2× PicoGreen working solution. The accuracy index is defined in Table 2. Calibrator MPO concentration was quantified with a commercially available assay (Human Myeloperoxidase Quantikine ELISA Kit, Bio-Techne, Minneapolis, MN) according to the manufacturer’s recommendations. Contents and Storage Information. Quant-iT ™ PicoGreen ™ dsDNA Assay Kit 1 mL assay kitAchieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. assay or add 5 µL Quant-iT ™ RiboGreen ™ RNA Reagent to 9. 0 ml 200x in DMSO b. Catalog Number. A Qubit dsDNA BR (broad range, 2 to 1000 ng) Assay Kit and Qubit dsDNA HS (high sensitivity, 0. • Specific for dsDNA in the presence of RNA. Quantification of cfDNA was performed using a TECAN Safire plate reader (96-well format) in combination with the Quant-iT ™ PicoGreen ™ dsDNA Assay Kit (Molecular Probes, Invitrogen; cat #P11496, lot #1911829) according to the manufacturer’s instructions, with slight modifications. 50 ul PicoGreen Mix by swirling bottle. Add µL of the Quant-iT™ PicoGreen® dsDNA Reagent (detailed in Section ) to each sample. The Quant-iT PicoGreen dsDNA. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. assay. Quant-iT PicoGreen dsDNA Assay Kit (1mL) Quant-iT PicoGreen dsDNA Reagent (1mL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P7589 P7581: Quant-iT PicoGreen dsDNA Assay Kit (10 x 100 µL) Quant-iT PicoGreen dsDNA Reagent (10 x 100 µL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P11496 P11495: Quant-iT OliGreen ssDNA Assay Kit Quant-iT OliGreen. 2. Using 20x TE buffer make 1x solution. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. Fluorescence signal measured for the DNA sodium salt standard from calf thymus using the Quant-iT™ PicoGreen® assay. Quant-iT™ PicoGreen® dsDNA Assay Kit *2,000 assays* (includes PicoGreen® Reagent, 10X buffer, and standard λ DNA) Quant-iT™ RiboGreen® RNA Reagent *2,000 assays* 10 ng/µl to 100 ng/µl RNA (>500 bp; not specific for RNA over DNA) RNase protection assays Microarray samplesQuant-iT PicoGreen dsDNA Assay Kit (10 x 100 µL) Quant-iT PicoGreen dsDNA Reagent (10 x 100 µL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P11496 P11495: Quant-iT OliGreen ssDNA Assay Kit Quant-iT OliGreen ssDNA Reagent: ssDNA : 200 pg–2 µg : 500/525 : 2,000 : O11492 O7582: Quant-iT RiboGreen RNA Assay Kit Quant-iT. ru by: 1. In the Quant-iT/PicoGreen assay, DNA and working solution were mixed 1:1. Quant-iT RNA BR (Broad Range) Assay Kit. Automation of the Quant-iT™ PicoGreen® dsDNA Assay Kit on the Fluent. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Picogreen kinetic assay was based on polymerase activity of SARS nsp12 in complex with nsp7L8, which. Using the Quant-iTTM PicoGreen® dsDNA reagent and the recommended assay protocol, researchers can quantitate dsDNA in the presence of equi molar concentrations of. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. 12. The PicoGreen reagent was discarded and the cells were rinsed with 4′,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich) solution for 10 min. Perform dsDNA quantification easily and quickly with Quant-iT dsDNA assay kits. Prepare a 1X TE working solution by diluting the concentrated buffer 20-fold with sterile, distilled, DNase-free water. This kit contains. Rapidly detect as little as 0. Genomic DNA was quantified using the Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions. 1 Prepare a 2 μ g/mL stock solution of dsDNA in TE. Application observe demonstrating so with Molecular Devices SpectraMax® microplate book and the Quant-iT PicoGreen assay, users can reliably size concentrations as low as 50 pg/mL of double-stranded DNA. 実験してみた – PicoGreenをプレートリーダーで使用してみた. Catalog Number. Quant-IT dsDNA Assay Kit, high sensitivity Quant-iT dsDNA Assay Kit, broad range Quant-iT 1X dsDNA high sensitivity; Target: dsDNA: dsDNA: dsDNA: Quantitation range: 0. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using the PicoGreen dsDNA quantitation assay and reagent. no. PicoGreen dsDNA quantitation reagents are. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. The assay is performed at room temperature, and the signal is stable for 3 hours. The Rapidly detect as little as 0. Reagent Search & Data Services Menu Reagent Search. Natural cures with herbs are. The assay is linear over three orders of magnitude and has little sequence. Within two years of follow-up, the primary endpoint defined as nonfatal myocardial infarction, stroke or transient ischemic attack, cardiovascular death, and >80% target vessel restenosis occurred in 34 patients (43%). Quantitation Range. 文件. Rapidly detect as little as 0. Nucleic acid quantitation using Quant-iT Reagents. In addition to the Quant-iT™ dsDNA High-Sensitivity Assay Kit described here, we also offer the Quant-iT ™ dsDNA Broad-Range Assay Kit (Cat. Q33130 Introduction The Quant-iT™ dsDNA Broad-Range Assay Kit makes DNA quantitation easy and. You will need 100 ul diluted PicoGreen per well containing 100 ul sample. pDNA encapsulation efficiency of LNP 10 and LNP 20 was measured by using a Quant-iT Pico-Green dsDNA assay kit (ThermoFisher Scientific,. Table 2 summarizes results of the. Sequencing was performed with a HiSeq Single-End Illumina platform (USA) [Citation 31]. 3. • Specific for dsDNA in the presence of RNA. 2 to 100 ng) Assay Kit were used with a Qubit 3. Rapidly detect as little as 0. Qubit DNA and RNA assays are available with different quantification ranges. the Quant-iT™ PicoGreen® dsDNA Assay Kits is certified to be nucleic acid–free and DNase-free. In case dsDNA standards are available only the quantification reagent can be used. 双链DNA(dsDNA)定量检测试剂盒 【产品升级声明】: 我司(懋康生物)从22. Thermo Fisher quant it picogreen dsdnaassay kit Quant It Picogreen Dsdnaassay Kit, supplied by Thermo Fisher, used in various techniques. The x-axis gives the mass of nucleic acid when DNA or RNA is assayed alone; in the 1:1 mixture, the total mass of nucleic acid is double. no. Note: The 20X TE buffer (Component B) included in the PicoGreen dsDNA Quantitation Kit is certified to be nucleic acid-free and DNase-free. Picogreen assays are compatible with most microplate readers and fluorometers. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. Components of the Quant-iT™ Picogreen™ dsDNA Assay Kit, including the Picogreen reagent and 100 µg/mL λDNA standard stock (Thermo Fisher Scientific), were utilized. DNA standard curve. ” application on Lunatic and compared to Qubit and. the Quant-iT™ PicoGreen® dsDNA Assay Kits is certified to be nucleic acid–free and DNase-free. Plasma levels. The Quant-iTTM PicoGreen® dsDNA Reagent is an ultra-sensitive fluorescent nucleic acid stain for quantitating double-stranded DNA (dsDNA) in molecular biology procedures. Blog May 11, 2021. Catalog Number Specifications Unit Size Product Type Quantitation Range Packaging Type Price (EUR) Availability Quantity;The assay was prepared for 184 aliquots of DNA sample and five DNA standards, including one blank. PicoGreen®試薬アッセイ. Quant-iT™ PicoGreen ® dsDNA Kit (Invitrogen, #P11496). Quantitation Range. Quant-iT™ PicoGreen ® dsDNA Reagent (10 vials, each containing 100 µL aliquots) 25 mL of 20X TE buffer; 1 mL of 100 µg/mL Lambda DNA (dsDNA standard) These jellies cheapest cialis uk and pills are can be ingested with or without food. 5 ng/ml of dsDNA were detected with minimal effect in the presence of ssDNA, RNA, and free nucleotides, which is comparable with InvitrogenTM Quant-iT™ PicoGreen® dsDNA Reagent. Quant-iT ™ 1X dsDNA Assay Kit, Broad-Range 4 ng−2 μg 1,000 reactions Q33267The supernatant containing the cytosolic extract was either immediately used or stored at − 80 °C until use. Picogreen assays are compatible with most microplate readers and fluorometers. P11495. PicoGreen is widely used in molecular biological procedures such as DNA fragment purification for subcloning, cDNA synthesis for library production as well as primer assays. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. 2 to 100 ng) Assay Kit were used with a Qubit 3. Katalognummer: P11495. 5 ng/mkl – 100 mg/ml. Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. using a PicoGreen assay by the company life technologies (Quant-iT™ PicoGreen dsDNA Reagent and Kits). Abstract. Rapidly detect as little as 0. • Specific for dsDNA in the presence of RNA. Components of the Quant-iT™ Picogreen™ dsDNA Assay Kit, including the Picogreen reagent and 100 µg/mL λDNA standard stock (Thermo Fisher Scientific), were utilized. Determination of DNA Yield. Quant-iT ™ 1X dsDNA Assay Kit, High Sensitivity 200 pg−100 ng 1,000 reactions Q33232 Quant-iT ™ 1X dsDNA Assay Kit, Broad-Range 4 ng−2 μg 1,000 reactions Q33267 Quant-iT ™ DNA Assay Kit, High Sensitivity 200 pg−100 ng 1,000 reactions Q33120 Quant-iT ™ DNA Assay Kit, Broad-Range 4 ng−1 μg 1,000 reactions Q33130 Quant-iT ™The Quant-iT™ dsDNA Broad-Range Assay Kit makes DNA quantitation easy and accurate. Triplicate 10 µL samples of DNA (), Escherichia coli rRNA or a 1:1 mixture of DNA and RNA were assayed with the Quant-iT dsDNA High-Sensitivity Assay Kit (). Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. 10pg/µl vs. The sample must yield a minimum of 55 ng of genomic DNA to ensure sufficient DNA for quality control (QC) and. Mix PicoGreen and DNA by pippetting up and down 5-10 times. Picogreen assays are compatible with most microplate readers and fluorometers. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA,. quant it picogreen dsdna assay kit reagents ( Thermo Fisher ) 86. Vortex solution briefly to mix well and centrifuge for 5 sec to bring liquid to bottom of tube; then dispense for use in the assay. Tweet. Product Type. • Suitable for use in 96- and 384-well plate formats. The SpectraMax Quant dsDNA Assay Kits provide pre-optimized, complete solutions that are sensitive, accurate, and easy to use. n = 3. Picogreen assays are compatible with most microplate readers and fluorometers. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. 作成者 LatB Staff 01. Quant-iT PicoGreen dsDNA Assay Kit 를 사용하면, ssDNA 와 RNA 그리고 free nucleotide 가 혼재되어 있는 시료에서도 dsDNA 만을 선택적으로 측정할 수 있으며, 25 pg /ml 의 매우 적은 양까지도 검출할 수 있습니다. PicoGreen dsDNA quantitation reagents are. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. Results: DNA concentration values were measured by the “DNA Mamm. Picogreen assays are compatible with most microplate readers and fluorometers. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. Working PicoGreen reagent was prepared by diluting the concentrated DMSO-PicoGreen stock solution, provided in the PicoGreen kit, 1:200 with TE according to the kit instructions. P11495. The DNA amount in each library was quantified with the Quant-iT PicoGreen dsDNA Assay Kit and with the Agilent 2200 TapeStation. The Qubit 1X dsDNA BR Standards includes standards at two concentrations, 0 and 100 ng/µL. Briefly, 2 µL of sample was diluted in 498 µL TE. 蛍光を用いたDNAの定量法の一つとしてInvitrogen™ Quant-iT™ PicoGreen™ dsDNA Assay Kit が利用されています。. 0 fluorometer (Selection Guide for the Qubit and Quant-iT Assay Kits. PCR products were quantified using the Quant-iT PicoGreen dsDNA Assay Kit on a Microplate reader (Bio-Tek, FLx800), and then mixed according to the amount of data required for each sample. P7589. BR reagent (Component A) 1. Tris-EDTA Buffer (10 mM tris, 1 mM EDTA), pH8. (Left) The Quant-iT dsDNA Assay Kit, High Sensitivity has a linear detection range of 0. Picogreen assays are compatible with most microplate readers and fluorometers. 1X dsDNA assay (%) 100 17. The Quant-iT family of assay kits—and the related Invitrogen Qubit family of assay kits designed for use with the Invitrogen Qubit 2. Rapidly detect as little as 0. lambda DNA standard 100ng/uL (in kit) PicoGreen reagent (in kit) 20x TE buffer (in kit) HPLC water; PicoGreen Prep. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. (The same tips can be used. This reagent is also included with the Quant-iT RiboGreen RNA Assay Kit and comes complete with buffer and RNA. The kits provide concentrated assay reagent, dilution buffer, and prediluted DNA standards. Simply dilute the reagent 1:200, load 200 µL into the wells of a microplate, add 1–20 µL sample, mix, then read the. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. 2–400ng/µl (1µl sample in 200µl assay). 1 mL kit. PicoGreen dsDNA quantitation reagent is a highly sensitive fluorescent nucleic acid stain which is used to quantitate double-stranded DNA. The Quant-iT dsDNA Broad-Range Kit is designed for assaying samples containing 2–1000 ng of DNA. The Quant-iT dsDNA Broad-Range Kit is designed for assaying samples containing 2–1000 ng of DNA. Picogreen assays are compatible with most microplate readers and fluorometers. samples as well as standard in the assay itself. Picogreen assays are compatible with most microplate readers and fluorometers. It requires the most user preparation and calculation, but can be adapted for cuvettes or plates. The Quant-iT dsDNA High-Sensitivity Assay Kit and the Quant-iT dsDNA Broad-Range Assay Kit make DNA quantification easy and accurate. . PicoGreen dsDNA quantitation reagents are. Quant It Picogreen Dsdna Assay Kit, supplied by Thermo Fisher, used in various techniques. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. Spectrofluorometry was performed based on guidelines from ThermoFisher Scientific Inc. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Rapidly detect as little as 0. • Suitable for use in 96- and 384-well plate formats. 95) in the range of 10 pg till 100 ng for the salmon dsDNA as well as for the control dsDNA from the kit (λ dsDNA standard) (Fig. Quant It Picogreen Dsdna Assay Kit Manual - 114,697 . 86100 stars. The superior sensitivity and performance of the Modulus™ Microplate Fluorometer allows for dsDNA detection as low as 250ng/mL. Unit Size. Approximate fluorescence excitation/emission maxima: 510/527 nm (see Figure 1, page 2) Quant-iT ™ dsDNA Broad-Range Assay Kit Catalog no. The SpectraMax Quant dsDNA Assay Kits provide pre-optimized, complete solutions that are sensitive, accurate, and easy to use. Prepare the 200 fold dilution of pico dye to be kept in the dark. 99. assay or add 5 µL Quant-iT ™ RiboGreen ™ RNA Reagent to 9. Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. Table 2 summarizes results of the. The following protocol is intended for the quantification of double-stranded DNA using Quant-iT™PicoGreen® dsDNA Assay Kit (ThermoFisher). Picogreen assays are compatible with most microplate readers and fluorometers. 2–100 ng, the fluorescence signal is linear with DNA. 10. The superior sensitivity and performance of the Modulus™ Microplate Fluorometer allows for dsDNA detection as. The limit of detection for the 20/20n is less than 45 pg in 100 µL total volume. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. No. No. Thermo Fisher Scientific公司的Quant-iT PicoGreen dsDNA 检测试剂盒对DNA更特异,比传统光吸收方法的灵敏度高约 1000倍。 如产品手册所述,此实验在微孔板格式中采用单 一染料浓度的动态范围从250 pg/mL到1000 ng/mL。在这 里,我们证明了使用Molecular Devices公司SpectraMax®Quant-iT PicoGreen dsDNA Assay Kit (10 x 100 µL) Quant-iT PicoGreen dsDNA Reagent (10 x 100 µL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P11496 P11495: Quant-iT OliGreen ssDNA Assay Kit Quant-iT OliGreen ssDNA Reagent: ssDNA : 200 pg–2 µg : 500/525 : 2,000 : O11492 O7582: Quant-iT RiboGreen RNA Assay Kit Quant-iT. Picogreen assays are compatible with most microplate readers and fluorometers. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. 99. 3. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. The reagent must be completely thawed before using it by bringing it to room temperature. Analysis of cfDNA and H3Cit was performed using Quant-iT™ PicoGreen ® dsDNA Assay Kit or an ELISA, respectively. 0 ml) of the aqueous working solution of Quant-iT™ PicoGreen dsDNA Reagent was added to each cell sample, which was incubated for 10 min at 37 °C in the dark. This sensitivity exceeds that achieved with the Hoechst 33258. Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. • Suitable for use in 96- and 384-well plate formats. 7 Q33232 4 Green features Less waste and use of fewer resources With the Qubit and Quant-iT 1X dsDNA HS and 1X dsDNA BR Assay Kits, instead of supplying the assay reagent and dilution buffer in separate bottles as was done with the. Antibodies Recombinant antibodies, monoclonal & polyclonal antibodies and primary & secondary antibodies. DNA. Picogreen assay kit (Invitrogen, P7589) a. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. The DeNovix dsDNA High. ZERO BIAS . The Quant-iT™ dsDNA Broad-Range Assay Kit makes DNA quantitation easy and accurate. • Specific for dsDNA in the presence of RNA. • Specific for dsDNA in the presence of RNA. Quantification and normalization of double stranded DNA with minimal set-up time. The assay is highly selective for double- The assay is highly selective for double- stranded DNA over RNA (see “Assay selectivity” on page 1), and in the range of 0. Q33130) Metabolic (cholesterol) • Amplex Red Cholesterol Assay Kit (Cat. The Quant-iT PicoGreen dsDNA Assay Kit from Thermo Fisher Scientific is more specific for DNA and is about 1000 times more sensitive than traditional absorbance methods. The assay is linear over three orders of magnitude and has little sequence dependence. 2023. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Quant-iT PicoGreen dsDNA Assay Kit. 5mL of TE x 5uL PicoReagent. Prepare the rest of the standards , to prepare enough working solution to assay 100 samples in 200 µL volumes, add 50 µL Quant-iT ™ RiboGreen RNA Reagent to 9. 1–100 ng: Ex / Em (nm) 502 / 523: 510 / 527: 502 / 523: Components: dsDNA HS fluorescent reagent, dilution buffer and standards RCA products may be directly quantified using the Quant-iT® PicoGreen® dsDNA Assay Kit or with a Qubit® Fluorometer after dilution to the appropriate detection range. 2– 100 ng Quant-iT 1X dsDNA HS Assay Kit – Quant-iT PicoGreen Reagent 1–200 ng Quant-iT OliGreen ssDNA Reagent and Assay Kit. 2. Rapidly detect as little as 0. 10. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. 1 mL kit. Picogreen Assays sind mit den meisten Mikrotiterplatten-Lesegeräten und Fluorometern kompatibel. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA,. The PG reagent from the Quant-iT TM PicoGreen TM dsDNA Assay Kit (Invitrogen) was prepared immediately before use by making a 1:200 dilution of the PG in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 7. All cfDNA were quantified by QUBIT dsDNA HS Assay kit (Thermo Fisher Scientific®, Aalst, Belgium), according to the manufacturer’s data. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using the PicoGreen dsDNA quantitation assay and reagent. Figure 2. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Table 3. Mit dem PicoGreen dsDNA. Measure concentration with a high-sensitivity assay (e. Microplate reader setup for a Quant-iT 1X dsDNA HS Assay. 04 corresponds to 2 μ g/mL dsDNA solution. DNA was quantified by the Quant-it PicoGreen dsDNA Assay Kit (Invitrogen Life technologies, UK) according to the manufacturer’s protocol. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. • Specific for dsDNA in the presence of RNA. In addition to conventional determination using 5 standards as present-ed by the manufacturer, we will show that reliable determi-nation is possible using only 2 standards. 95 mL TE for the . Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. 2. 5 ng/µL. Description. The fusion protein nsp7-nsp8 was generated by inserting a GSGSGS linker sequence. Breadcrumb. Abstract. Quant-iT™ PicoGreen® dsDNA Assay Kit From Molecular Probes (Invitrogen) May 22, 2007. quant it picogreen dsdna assay kit reagents ( Thermo Fisher ) 86. Table 2 summarizes results of the. Related applications: Nucleic Acid Quantitation. Quant-iT PicoGreen dsDNA Assay Kit a. (A) The overall set up of the assay includes manual preparation of the reagents, automated preparation of the assay plate and fluorometric quantification using a plate reader. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. SpectraMax Plus Spectrophotometer (Molecular Devices) and Picogreen quantitation was performed using Quant-iT™ PicoGreen® dsDNA Assay Kit From Molecular Probes (Invitrogen). Prepare a 1X TE working solution by diluting the concentrated buffer 20-fold with sterile,Calibrator DNA content was assessed with Quant-iT™ PicoGreen™ dsDNA Assay Kit (Thermo Fisher Scientific). Triplicate 10 µL samples of DNA (), Escherichia coli rRNA or a 1:1 mixture of DNA and RNA were assayed with the Quant-iT dsDNA High-Sensitivity Assay Kit (). 5 ng/mL 990 10 1000 250 pg/mL 999 1 1000 25 pg/mL 1000 0 1000 Blank Figure 1. 2023. The QFX is a powerful solution for all fluorometric applications where enhanced specificity or sensitivity are needed. Regulations & Guidance; Manuals 0 0. PicoGreen assay. Optimized for fluorescence microplate readers. This protocol is a simplified and condensed version of. Quant It Picogreen Dsdna Assay Kit Reagents, supplied by Thermo Fisher, used in various techniques. Equal amounts (100 µL) of working PicoGreen quantitation reagent were mixed and incubated for 10 minutes at room temperature, protected from light. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. dsDNA standards were incubated in cuvettes and measured using a Varian Cary Eclipse. このアッセイは、従来のUV吸光度法に比べて感度が何倍も高いため、様々な濃度範囲に対して最適化することが可能です。. PicoGreen reagent provides rapid and easy determination of dsDNA, offering a wide dynamic range. Prepare the assay. Perform dsDNA quantification easily and quickly with Quant-iT dsDNA assay kits. For example, 3 µL of the DNA standard mixed with 147 µL of TE is sufficient for the standard curve described in step 2. Invitrogen™ Quant-iT™ PicoGreen™ dsDNA Assay Kits and dsDNA Reagents. • Suitable for use in 96- and 384-well plate formats. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using the PicoGreen dsDNA quantitation assay and reagent. PicoGreen定 量检测方法简单、方便,被多家生物制品厂所选择,成为生物制品残留DNA检测的标准。 PicoGreen与DNA双链结合后才发出的荧光,无DNA不发荧光;所发荧光与DNA浓度成 正比。在2010年《中国药典》中提出,PicoGreen定量DNA的方法检出限约0. Q33130). Catalog Number. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. Calibrator DNA content was assessed with Quant-iT™ PicoGreen™ dsDNA Assay Kit (Thermo. In addition to the Quant-iT™ dsDNA Broad-Range Assay Kit described here, we also offer the Quant-iT™ dsDNA High-Sensitivity Assay Kit (Cat. Picogreenアッセイは、ほとんどのマイクロプレートリーダーおよび蛍光光度計に. 20pg/µl for Quant-iT PicoGreen® (1µl of sample in a 200µl assay volume). The PicoGreen reagent was freshly prepared by diluting 15 μL reagent with 2 mL of TE buffer (~ 133X) and stored in the dark for no longer than 2 hours. The DeNovix dsDNA Broad Range Assay is ideal for measuring sample concentrations ranging from 0. The Quant-iT™ Picogreen® double stranded DNA reagent is added and mixed with both the samples and references through a controlled aspiration and dispense. dsDNA만을 선택적으로 정량. 5 ng/mL 990 10 1000 250 pg/mL 999 1 1000 25 pg/mL 1000 0 1000 Blank Figure 1. Product Type. Unlike many other DNA quantitation methods, PicoGreen is minimally affected by the presence of lipid, protein, carbohydrates and other cellular. The Quant-iT™ PicoGreen® dsDNA Assay Kit makes for an easy, reliable, and accurate method to quantify dsDNA samples when used in conjunction with the Modulus™ Microplate Fluorometer from Turner BioSystems, Inc. Quant-iT PicoGreen dsDNA Assay Kit (1mL) Quant-iT PicoGreen dsDNA Reagent (1mL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P7589 P7581: Quant-iT PicoGreen dsDNA Assay Kit (10 x 100 µL) Quant-iT PicoGreen dsDNA Reagent (10 x 100 µL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P11496 P11495: Quant-iT OliGreen ssDNA Assay Kit Quant-iT OliGreen. 2–100 ng and is selective for dsDNA, even in the presence of an equal mass of RNA. 0. Absorbance-based measurements employ the DNA as it is and volumes of 100 µl, 50 µl, and 2 µl were placed into a 96 well, 384 well, or LVis plate, respectively. Picogreenアッセイは、ほとんどのマイクロプレートリーダーおよび蛍光光度計に. 参考資料. Rapidly detect as little as 0. Prepare a 1X TE working solution by diluting the concentrated buffer 20-fold with sterile, distilled, DNase-free water. Rapidly detect as little as 0. To perform fluorometric quantification of cfDNA quantity Quant-iT PicoGreen dsDNA Assay Kit (Thermofisher), was used, following the manufacturer’s instructions. • Suitable for use in 96- and 384-well plate formats. Picogreen assays are compatible with most microplate readers and fluorometers. Quant-iT PicoGreen dsDNA AssayキットおよびdsDNA試薬を使用すると、幅広いダイナミックレンジでdsDNA濃度測定を正確かつ精密に行うことができます。. ZERO BIAS - scores, article reviews, protocol conditions and moreGot it. QuantiFluor® ONE dye sensitivity range is 0. The PicoGreen dsDNA quantitation reagent and kits are ideal for PCR-based assays, microarray samples, DNA damage assays, enzyme activity assays, genomic DNA. PicoGreen®試薬アッセイは、蛍光を基にした迅速かつ簡単な手法により、dsDNAを正確に定量することができます。. Total amount of 1X TE per assay will be 200 μl per well which includes the amount of TE used to dilute the PicoGreen reagent. We quantified the extract with Quant-iT™ PicoGreen™ dsDNA Assay Kits and dsDNA Reagents (Thermos Fisher Scientific, Waltham, MA, USA) to evaluate the amount of residual DNA. Technical note | Quant-iT and Qubit 1X dsDNA assays the Quant-iT™ PicoGreen® dsDNA Assay Kits is certified to be nucleic acid–free and DNase-free. The assay is linear over three orders of magnitude and has little sequence dependence, allowing you to accurately measure DNA from many sources, including genomic DNA, viral DNA. The reagent Quant-iT PicoGreen was added to all wells and the samples were incubated for 5 min at room. The tubes were. Katalognummer: P11496. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read.